Fast processing of microscopic images using object-based extended depth of field
نویسندگان
چکیده
منابع مشابه
Noise removal in extended depth of field microscope images through nonlinear signal processing.
Extended depth of field (EDF) microscopy, achieved through computational optics, allows for real-time 3D imaging of live cell dynamics. EDF is achieved through a combination of point spread function engineering and digital image processing. A linear Wiener filter has been conventionally used to deconvolve the image, but it suffers from high frequency noise amplification and processing artifacts...
متن کاملExtended Depth of Field
I n t roduc t ion The narrow depth of field of the light microscope is either an asset or a liability depend microscopist. Occasionally, by limiting the image to a narrow plane in the vertical axis, i observer’s attention by isolating structures in a thick sample, perm object while ignoring distracting elements abov cases, this feature is a liability when the object’s importance lies in its thr...
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a novel and ultra low cost isfet electrode and measurement system was designed for isfet application and detection of hydronium, hydrazinium and potassium ions. also, a measuring setup containing appropriate circuits, suitable analyzer (advantech board), de noise reduction elements, cooling system and pc was used for controlling the isfet electrode and various characteristic measurements. the t...
High depth of field microscopic imaging using an interferometric camera.
We describe the design of a microscope combining rotational shear interferometer (RSI)-based coherence imaging with an objective lens to simultaneously obtain high numerical aperture and high depth of field imaging. We present experimental results showing the operation of this instrument.
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The graph cut based approach has become very popular for interactive segmentation of the object -of-interest from the background. Content-based multimedia application plays an important role on automatic segmentation of images with low depth of field (DOF).Graph cut method, separate the important objects (i.e. interest regions) of a given image from its defocused background using novel cluster ...
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ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2016
ISSN: 1471-2105
DOI: 10.1186/s12859-016-1373-2